Activation of the adenovirus major late promoter by transcription factors MAZ and Sp1
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چکیده
منابع مشابه
Activation of the adenovirus major late promoter by transcription factors MAZ and Sp1.
Multiple binding sites for the transcription factors MAZ and Sp1 within the adenovirus type 5 major late promoter have been identified by DNase I protection studies. In the proximal region of the promoter, both MAZ and Sp1 interact with GC-rich sequences flanking the TATA box. Two MAZ binding sites are centered at -18 and -36 relative to the transcriptional initiation site. Sp1 bound only to th...
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The rat phenylethanolamine N-methyltransferase (PNMT) gene promoter contains 1-base pair (bp) overlapping consensus sequences for Sp1 and MAZ transcription factors at -48 and -38 bp, respectively. Gel mobility assays using PC-12-derived RS1 cell nuclear extracts or in vitro translated proteins showed that Sp1 and MAZ specifically bind to these elements, that MAZ displaces/prevents Sp1 binding, ...
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Monoclonal antibody directed against RNA polymerases IIO and IIA completely inhibits specific transcription from the major late promoter of adenovirus-2. At comparable antibody concentrations, only a slight inhibition of polymerase III transcription of VA RNA I is observed. Inhibition of polymerase II transcription is specific for monoclonal IgM directed against RNA polymerase II. High concentr...
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A simple approach has been developed for the unambiguous identification and purification of sequence-specific DNA-binding proteins solely on the basis of their ability to bind selectively to their target sequences. Four independent methods were used to identify the promoter-specific RNA polymerase II transcription factor MLTF as a 46-kilodalton (kDa) polypeptide. First, a 46-kDa protein was spe...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1997
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.71.12.9600-9607.1997